Journal: Pharmacology, biochemistry, and behavior

Article Title: Stress induces behavioral sensitization, increases nicotine-seeking behavior and leads to a decrease of CREB in the nucleus accumbens.

doi: 10.1016/j.pbb.2012.01.025

Figure Lengend Snippet: Fig. 7. (A) Representative membrane with fluorescent bands from Western blots of ERK and phosphoERK (CS: control-saline; CN: control-nicotine; SS: stress-saline; SN: stress- nicotine). (B) Western blotting analysis of total ERK and phosphoERK in the nucleus accumbens in samples collected after locomotor activity tests. Data represent ±SEM of 9–10 animals per group.

Article Snippet: ERK activity levels were assessed using antibodies against total ERK (1:2000; Cat# 9102; Cell Signaling Technology, Danvers, MA, USA) and phospho ERK (1:1000; Cat# 7383; Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Membrane, Western Blot, Control, Saline, Activity Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Signaling through mutants of the IgA receptor CD89 and consequences for Fc receptor gamma-chain interaction.

doi: 10.4049/jimmunol.176.6.3603

Figure Lengend Snippet: FIGURE 5. FcRI cross-linking triggered MAPK phosphorylation. Cells were incubated with an anti-FcRI mAb and cross-linked with goat F(ab)2 anti-mouse IgG1 for 20 s (20) to 20 min (20) to induce FcRI- mediated MAPK phosphorylation. As a negative control, cells were only incubated with anti-FcRI mAb ( ) or no Ab (n.ab). As a positive control, cells were incubated with goat F(ab)2 anti-mouse IgG for 2 to trigger the BCR (). For each cell line, anti-phospho-MAPK staining is shown in the upper panels, and total MAPK is shown in the lower panels (n 2).

Article Snippet: Membranes were blocked in 5% BSA (Roche) and probed with anti-phospho-p44/42 MAPK or anti-total MAPK Ab for 2 h (Cell Signaling Technology).

Techniques: Phospho-proteomics, Incubation, Negative Control, Positive Control, Staining